Seidel, Thorsten; Schnitzer, Daniel; Golldack, Dortje; Sauer, Markus; Dietz, Karl-Josef: Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis. In: BMC Cell Biology. Jg.9 H. 1. 2008
Inhalt
- Results
- In silico sequence analysis
- Transcript analysis of proteolipid subunits
- Subcellular localisation
- Stoichiometry of VHA-e and VHA-c"
- FRET between transmembrane VHA-proteolipid subunits
- FRET between proteolipid subunits-CFP-fusions and VHA- a-YFP
- FRET efficiency between VHA-e-CFP and the acceptors VHA-a-YFP, VHA-c-YFP and VHA-c"-YFP
- FRET efficiency between VHA-a-YFP and VHA-A-CFP
- Discussion
- Conclusion
- Methods
- Materials
- Molecular biology
- In silico analysis
- Analysis of the transcript level
- Isolation and transfection of mesophyll protoplasts
- Confocal laser scanning microscopy
- Investigation of subcellular localisation
- FRET measurements
- Data processing
- Bimolecular fluorescence complementation
- List of abbreviations
- Authors' contributions
- Additional material
- Acknowledgements
- References