Shaikhali, Jehad; Heiber, Isabelle; Seidel, Thorsten; Stroeher, Elke; Hiltscher, Heiko; Birkmann, Stefan; Dietz, Karl-Josef; Baier, Margarete: The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes. In: BMC Plant Biology. Jg.8 H. 1. 2008, S. 48 ff.
Inhalt
- Results
- Isolation of Rap2.4a
- Localization of the Rap2.4a-binding site in the 2CPA promoter
- In vivo function of C3G4 in Rap2.4a-regulation of 2CPA transcription
- Localization of Rap2.4a in Arabidopsis mesophyll protoplasts
- Redox regulation of Rap2.4a-dependent 2CPA transcription
- Redox regulation of Rap2.4a protein
- Effect of Rap2.4a deletion on gene expression using T- DNA insertion lines
- Effect of Rap2.4a disruption on environmental stability
- Discussion
- Conclusion
- Methods
- Plant material and growth conditions
- One-hybrid screen of Arabidopsis thaliana cDNA library
- RNA isolation and RT-PCR
- Polyacrylamide gel electrophoresis (PAGE)
- Expression and purification of recombinant proteins
- Electrophoretic mobility shift assays (EMSA)
- Analysis of the DNA-binding affinity of Rap2.4a
- Mutagenesis of the 2CPA promoter and transient expression analysis
- Fluorometric transactivation assays and localization studies
- Luminometric transactivation assays
- Redox titration
- Identification and isolation of T-DNA insertion mutants
- Accession numbers
- List of abbreviations
- Authors' contributions
- Acknowledgements
- References