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Wormuth, Dennis; Baier, Margarete; Kandlbinder, Andrea; Scheibe, Renate; Hartung, Wolfram; Dietz, Karl-Josef: Regulation of gene expression by photosynthetic signals triggered through modified CO2 availability. In: BMC PLANT BIOLOGY. Jg.6 H. 1. 2006
Inhalt
Results
Metabolic regulation
The energy status
Assimilatory force and the reduction state of the NADP system
Antioxidant protection
Ascorbate contents and redox states
Glutathione contents and redox states
Activities of antioxidant enzymes
Contents of soluble and hydrolysable sugars
ABA levels in ambient air
Photosynthetic performance of wild-type and mutants
Transcript level regulation in response to high and low CO2
Discussion
Regulation of ROS-responsive genes
Ascorbate-dependent regulation
Coupling of transcript abundance regulation
Correlation with the adenylate status
Conclusion
Methods
Plant material and growth conditions
Metabolite analysis
Chlorophyll-a-fluorescence measurements
Determination of protein contents and biochemical analyses of enzyme activities
RNA isolation, cDNA synthesis and RT-PCR analysis
Statistical analysis
Abbreviations
Authors' contributions
Acknowledgements
References