Rückert, Christian ; Koch, DJ; Rey, DA; Albersmeier, Andreas; Mormann, S; Pühler, Alfred ; Kalinowski, Jörn: Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction. In: BMC Genomics. Jg.6 H. 1. 2005
Inhalt
- Background
- Results
- Identification of a Corynebacterium glutamicum gene cluster possibly involved in assimilatory sulphate reduction by sequence similarity and comparative genomics
- Bioinformatic analysis of the Corynebacterium glutamicum candidate genes possibly involved in assimilatory sulphate reduction
- Comparison of the putative sulphate assimilation gene cluster of Corynebacterium glutamicum with gene clusters of other members of the actinomycetes
- Characterisation of the Corynebacterium glutamicum gene cluster possibly involved in assimilatory sulphate reduction by targeted gene deletion, mutational analysis, and complementation
- Analysis of the Corynebacterium glutamicum gene cluster fpr2cysIXHDNYZ by growth tests in liquid culture
- Transcriptional analysis of the Corynebacterium glutamicum cys gene cluster
- Determination of the transcription start sites in front of the Corynebacterium glutamicum fpr2 and cysI genes
- Discussion
- Conclusion
- Methods
- Bacterial strains, plasmids and culture media
- DNA isolation, transfer and manipulation
- Construction of plasmids
- Site-specific gene deletion
- Identification of a cysI transposon mutant
- Relative quantification of mRNA levels using real-time RT- PCR
- Determination of transcriptional starts with the RACE method
- Real-time monitoring of cell growth using nephelometry
- GenBank/TrEMBL accession numbers
- Bioinformatic analysis
- Authors' contributions
- Additional material
- Acknowledgements
- References