Hellweg, Christoph; Pühler, Alfred ; Weidner, Stefan: The time course of the transcriptomic response of Sinorhizobium meliloti 1021 following a shift to acidic pH. In: BMC MICROBIOLOGY. Jg.9 H. 1. 2009, S. 37 ff.
Inhalt
- Background
- Results and Discussion
- Growth analysis of S. meliloti 1021 cultures exposed to neutral and acidic pH
- Cluster analysis of expression profiles of S. meliloti genes following a shift to acidic pH
- Most of cluster A genes encode proteins carrying signal peptides
- The large cluster B contains some exo genes responsible for the biosynthesis of succinoglycan and several rpoE2 dependently regulated genes
- Cluster C contains among others genes coding for a chaperone and a component of a low O2 affinity oxidase
- Cluster D comprises carbon uptake and fatty acid degradation genes
- Cluster E contains genes involved in nitrogen metabolism, ion transport and methionine metabolism
- Cluster F is almost exclusively composed of genes playing a role in chemotaxis and motility
- Cluster G consists of several genes involved in nitrogen uptake and utilization
- Cluster H is formed by genes with distinct biological functions and a high variation in their expression levels
- The time resolved expression profile of the S. meliloti 1021 exo genes and flagellar genes following a shift to acidic pH
- Conclusion
- Methods
- Media and growth conditions
- RNA isolation
- Transcriptional profiling using the SM6kOligo whole genome microarray
- Filtering and clustering analysis of the microarray data
- Authors' contributions
- Additional material
- Acknowledgements
- References