Hansmeier, Nicole; Bartels, Frank Wilco; Ros, Robert; Anselmetti, Dario ; Tauch, Andreas; Pühler, Alfred ; Kalinowski, Jörn: Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. In: J Biotechnol. Jg.112 H. 1-2. 2004, S. 177-193
Inhalt
- Bacterial strains and growth conditions
- Extraction of S-layer proteins from C. glutamicum
- Separation and identification of PS2 proteins by SDS-PAGE and MALDI-TOF-MS
- PCR amplification, cloning and sequencing of C. glutamicum S-layer genes
- Bioinformatics tools used to analyze nucleotide and amino acid sequences
- Atomic force microscopy of S-layer proteins
- Results
- Identification of surface-layer proteins of 28 different C. glutamicum isolates
- Sequence analysis of S-layer genes isolated from 28 different C. glutamicum isolates
- Classification of C. glutamicum S-layer proteins based on sequence similarities
- The C. glutamicum S-layers of the five identified classes are characterized by hexagonal structures with a variable unit cell dimension
- Discussion
- Comparative sequence analyses revealed hyper-variable regions within the C. glutamicum S-layer proteins
- C. glutamicum S-layers display a common hexagonal structure with a varying unit cell dimension which correlates to a sequence similarity-based classification scheme
- Acknowledgements
- References