Mormann, Sascha; Lömker, Alexander; Rückert, Christian ; Gaigalat, Lars; Tauch, Andreas; Pühler, Alfred ; Kalinowski, Jörn: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis [...]. In: BMC Genomics. Jg.7 H. 1. 2006
Inhalt
- Background
- Results
- Transposon mutagenesis of Corynebacterium glutamicum ATCC 13032 with an IS6100-based transposon vector
- Auxanographic screening and targeted gene identification using PCR techniques
- Identification of a novel C. glutamicum gene closing the last gap in histidine biosynthesis
- Validation of cg0910 gene function by deletion and homologous complementation
- Phylogenetic analysis of Cg0910 and related inositol monophosphatases (IMP)
- Discussion
- Conclusion
- Methods
- Bacterial strains, plasmids, oligonucleotides and culture conditions
- DNA isolation, manipulation and transfer
- Transformation of C. glutamicum
- Determination of transposon integration sites
- Sequence data/accession numbers
- Bioinformatic analyses and tools for interpretation of C. glutamicum sequences
- Auxotrophy screening and supplementation of auxotrophic phenotypes
- PCR techniques and conditions
- Deletion and genetic complementation of cg0910
- Authors' contributions
- Acknowledgements
- References