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Nematollahi, Ghazaleh; Kianianmomeni, Arash; Hallmann, Armin: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. In: BMC Genomics. Jg.7 H. 1. 2006
Inhalt
Results
Target genes for differential analysis
Characteristics of genes within subset A
Characteristics of genes within subset B
Characteristics of genes within subset C
Characteristics of genes within subset D
Characteristics of genes within subset E (novel Volvox genes)
Characteristics of genes within subset F (novel Volvox genes)
Characteristics of genes within subset G (novel Volvox genes)
Selection of primer combinations and testing by genomic PCR and RT-PCR
Analysis of cell-type specific gene expression by real-time RT-PCR experiments
Discussion
Gene subsets A-C: comparison of the obtained real-time RT-PCR results with the expectations
Gene subsets D-G: validation of the obtained real-time RT- PCR results
Conclusion
Methods
Culture conditions
Sequence analysis and homology search
Primer design
Large-scale separation of cell types
Isolation of total RNA
Isolation of genomic DNA
Genomic PCR
Standard RT-PCR
Real-time RT-PCR
Analysis of gene expression by using the 2-DDCt method
GenBank accession numbers
Abbreviations
Authors' contributions
Acknowledgements
References