Kleindt, Christiane Katja; Stracke, Ralf ; Mehrtens, Frank; Weisshaar, Bernd: Expression analysis of flavonoid biosynthesis genes during Arabidopsis thaliana silique and seed development with a primary focus on the proanthocyanidin [...]. In: BMC Research Notes. Jg.3 H. 1. 2010
Inhalt
- Results and Discussion
- Correlation of silique length to embryo developmental stages and timing of proanthocyanidin accumulation in the testa during seed development
- Verification of silique and seed developmental series ATH1 GeneChip data by quantitative real time PCR
- Evaluation of specificity of isolated seed, valve and replum organs by organ-specific expression analysis
- Reanalysis of the ATH1 silique and seed developmental series reveals stage-specific expression patterns of flavonoid biosynthesis genes
- Expression of flavonoid biosynthesis genes is seed-specific
- TT2 and TT8 show organ-specific gene expression in isolated seeds
- Genes involved in anthocyanin accumulation are activated during early stages of seed development
- Methods
- Plant growth conditions
- Harvest and stage confirmation of plant material for Affymetrix GeneChip experiments
- RNA extraction for Affymetrix GeneChip experiments
- Affymetrix GeneChip hybridization, data pre-processing and expression analyses
- Harvest of plant material, RNA extraction and cDNA synthesis for qPCR experiments
- qPCR and data analyses
- Vanillin assay
- Acknowledgements
- Author details
- Authors' contributions
- Competing interests
- References