Höhn, Stephanie; Hallmann, Armin: There is more than one way to turn a spherical cellular monolayer inside out: type B embryo inversion in Volvox globator. In: BMC Biology. Jg.9 H. 1. 2011
Inhalt
- Results
- Major movement patterns of the cellular monolayer
- Pre-inversion stage
- Early inversion stage
- Mid-inversion stage
- Late inversion stage
- Early and late post-inversion stage
- Region and stage-specific changes in cell shape
- Pre-inversion stage
- Early inversion stage
- Mid-inversion stage
- Late inversion stage
- Early and late post-inversion stage
- Region- and stage-specific changes in the locations of CBs
- Pre-inversion stage
- Early inversion stage
- Mid-inversion stage
- Late inversion and post-inversion stage
- Changes in shapes of nuclei, growth of flagella and biosynthesis of extracellular matrix
- Modeling of cell shapes, CB locations and illustration of the entire inversion process
- Discussion
- Comparison of the type A and type B inversions
- Evolution of inversion
- Biomechanical possibilities and implications about the involved tensile and compressive forces: a global mechanistic scenario
- Conclusions
- Methods
- Strain and culture conditions
- High-resolution in vivo stereo LM
- Microsurgery
- Fixation and dehydration of embryos
- Embedding and sectioning
- Staining
- Sputter coating
- LM of sections
- TEM
- SEM
- cLSM
- Two-dimensional and 3D modeling
- Acknowledgements
- Authors' contributions
- References