Schwientek, Patrick; Szczepanowski, Rafael; Rückert, Christian ; Kalinowski, Jörn; Klein, Andreas; Selber, Klaus; Wehmeier, Udo F.; Stoye, Jens ; Pühler, Alfred: The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110. In: BMC Genomics. Jg.13 H. 1. 2012
Inhalt
- Results and discussion
- High-throughput pyrosequencing and annotation of the Actinoplanes sp. SE50/110 genome
- General features of the Actinoplanes sp. SE50/110 genome
- Phylogenetic analysis of the Actinoplanes sp. SE50/110 16S rDNA reveals highest similarity to Actinoplanes utahensis
- Comparative genome analysis reveals 50% unique genes in the Actinoplanes sp. SE50/110 genome
- The high quality genome sequence of Actinoplanes sp. SE50/110 corrects the previous sequence and annotation of the acarbose biosynthetic gene cluster
- Several genes of the acarbose gene cluster are also found in other locations of the Actinoplanes sp. SE50/110 genome sequence
- The Actinoplanes sp. SE50/110 genome hosts an integrative and conjugative element that also exists in multiple copies as an extrachromosomal element
- Four putative antibiotic production gene clusters were found in the Actinoplanes sp. SE50/110 genome sequence
- Conclusions
- Methods
- Cultivation of the Actinoplanes sp. SE50/110 strain
- Preparation of genomic DNA
- High throughput sequencing and automated assembly of the Actinoplanes sp. SE50/110 genome
- Construction of a fosmid library for the Actinoplanes sp. SE50/110 genome finishing
- Terminal insert sequencing of the Actinoplanes sp. SE50/110 fosmid library
- Finishing of the Actinoplanes sp. SE50/110 genome sequence by manual assembly
- Prediction of open reading frames on the Actinoplanes sp. SE50/110 genome sequence
- Functional annotation of the identified open reading frames of the Actinoplanes sp. SE50/110 genome
- Phylogenetic analyses
- Acknowledgements
- Author details
- Authors' contributions
- Competing interests
- References