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Cavalcanti de Lucena, Daniella Karine: Characterization of sigma factors of Sinorhizobium meliloti involved in stress response. 2011
Inhalt
2.1 Heat shock07
2.2 Cold shock08
2.3 Oxidative stress010
2.4 pH stress 011
2.5 Other stresses0 11
1.9 Buffers and solutions034
2.5.2 Osmotic Stress
Salt stress is one of the major environmental stress conditions and constitutes a severe problem facing the establishment of bacteria in their natural environment. Survival and growth in saline environments are the result of adaptive processes, such a...
1.9 Buffers and solutions
Solutions for microarray hybridizations
Buffer for the preparation of competent E. coli cells
2.5.1 Isolation of genomic DNA from S. meliloti
Checking fluorescently labeled targets on agarose gels prior to microarray hybridizations
2.7.2 Microarray data analysis
Pre-processing
Normalization and filtering
Clustering analysis of the microarray data