Hoffman, Joseph ; Thorne, Michael As; Trathan, Philip N; Forcada, Jaume: Transcriptome of the dead: characterisation of immune genes and marker development from necropsy samples in a free-ranging marine mammal. In: BMC Genomics. Jg.14 H. 1. 2013
Inhalt
- Background
- Results
- RNA yields from necropsy samples
- Sequence data generated
- Transcriptome assemblies
- Functional annotation
- Comparative genomics between the fur seal and dog
- Molecular marker discovery
- Discussion
- Conclusions
- Methods
- Animal sampling
- RNA isolation and cDNA generation
- Skin transcriptome data
- Sequence assembly
- BLAST mapping and sequence annotation
- Comparative genomics with the dog
- Mining for molecular markers
- Availability of supporting data
- Additional files
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References