Bogen, Christian; Al-Dilaimi, Arwa; Albersmeier, Andreas; Wichmann, Julian; Grundmann, Michael; Rupp, Oliver; Lauersen, Kyle J. ; Blifernez-Klassen, Olga; Kalinowski, Jörn; Goesmann, Alexander ; Mussgnug, Jan H.; Kruse, Olaf: Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel [...]. In: BMC Genomics. Jg.14 H. 1. 2013
Inhalt
- Results
- M. neglectum exhibits a rapid photoautotrophic growth phenotype and accumulates neutral lipids to a high extent under nitrogen starvation
- M. neglectum shows a robust growth phenotype with a high salt and pH tolerance during phototrophic growth
- Full genome sequencing and assembly reveals the diploid character of the M. neglectum genome
- Genome annotation identifies M. neglectum as member of the Selenastraceae with a large mitochondrial genome
- Comparative analyses of the predicted gene functions revealed higher gene numbers assigned to carbohydrate metabolism and fatty acid biosynthesis
- Detailed genome analysis based on lipid pathway reconstruction provides essential new insights into the lipid metabolism of green microalgae
- Discussion
- The characterization of M. neglectum as a robust phototrophic oleaginous strain highlights its potential suitability for oil-based biofuel production
- The genome of M. neglectum reveals a highly diverse repertoire of genes encoding catalytic activities in carbohydrate and fatty acid metabolism
- The reconstruction of lipid pathways in M. neglectum reveals new insights into fatty acid and neutral lipid synthesis
- M. neglectum can serve as a model organism to elucidate the distinct role of PDAT enzymes in TAG accumulation and homeostasis
- Conclusions
- Methods
- Cultivation conditions
- Nitrogen starvation and stress tolerance
- Lipid extractions and chromatographies
- FAME analysis
- DNA isolation and sequencing
- Genome assembly and gene annotation
- Phylogenetic analysis
- Metabolic pathway reconstruction
- Additional files
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- References