Eickenberg, Bernhard: Superparamagnetic beads as self-assembling matter for microfluidic applications. 2014
Inhalt
- 1 Introduction
- 2 Theory
- 2.1 Microfluidics
- 2.1.1 From the macroscopic to the microscopic
- 2.1.2 Fluid flow on the micro scale
- 2.1.3 Pressure-Driven Flow Profile
- 2.1.4 Mixing on the micro scale
- 2.2 Magnetism
- 2.3 Optical Detection
- 3 Materials and Methods
- 3.1 Lithography
- 3.2 Microfluidic Flow
- 3.3 Optical Microscopy
- 3.4 Magnetic Field
- 3.5 Raman Spectroscopy
- 3.6 Superparamagnetic Beads
- 4 SPS Formation Dynamics
- 4.1 Materials and Methods
- 4.2 Experiments
- 4.2.1 Evaluation of the Initial SPS Formation
- 4.2.2 Evaluation of the Frequency Threshold
- 4.2.3 Reformation of Chain-like SPS from Clusters
- 4.3 Results and Discussion
- 4.3.1 Evaluation of the Initial SPS Formation
- 4.3.2 Evaluation of the Frequency Threshold
- 4.3.3 Reformation of Chain-like SPS from Clusters
- 4.4 Conclusion
- 4.5 Outlook
- 5 Utilizing SPS structures for colloidal separation and enhanced mixing
- 5.1 Materials and Methods
- 5.2 Experiments
- 5.2.1 Guiding and Separation Efficiency
- 5.2.2 Mixing Efficiency
- 5.2.3 Free-Flowing SPS for Particle Separation
- 5.3 Results and Discussion
- 5.3.1 Guiding and Separation Efficiency
- 5.3.2 Free-Flowing SPS for Particle Separation
- 5.3.3 Mixing Efficiency
- 5.3.4 Comparison with Magnetophoresis
- 5.4 Conclusion
- 5.5 Outlook
- 6 DNA Bridges as Bead-Bead Interconnections
- 6.1 Materials and Methods
- 6.2 Experiments
- 6.2.1 Binding DNA to the Bead Surface
- 6.2.2 Monolayer Formation
- 6.2.3 Evaluation of the DNA Binding Strength
- 6.3 Results and Discussion
- 6.3.1 Binding DNA to the Bead Surface
- 6.3.2 Monolayer Formation
- 6.3.3 Evaluation of the DNA Binding Strength
- 6.4 Conclusion
- 6.5 Outlook
- 7 SPS chains as Switchable Filtration Network
- 8 Beads for Surface Enhanced Raman Spectroscopy
- 9 Summary
- Bibliography