Schürmann, Matthias; Wolff, Annalena; Widera, Darius ; Hauser, Stefan; Heimann, Peter; Hütten, Andreas; Kaltschmidt, Christian; Kaltschmidt, Barbara: Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation. In: Stem Cell Research. Jg.13 H. 1. 2014, S. 98-110
Inhalt
- Materials and methods
- Generation of a nanoporous titanium surface
- Isolation and cultivation of neural crest-derived human ITSCs
- Immunocytochemistry and staining for F-actin
- Osteogenic differentiation via biochemical cues
- Osteogenic differentiation via topological cues
- Detection of calcium deposition (Alizarin red S and Von Kossa staining)
- Calcium assay (Alizarin Red S dye recovery)
- RNA isolation, primer design and qRT-PCR
- Scanning electron microscopy
- Proliferations and viability assay
- Statistical analysis
- Results
- Human neural crest-derived ITSCs form neurospheres and express stem cell marker nestin
- Cultivated ITSCs are able to differentiate into osteogenic lineage after cultivation in the presence of biochemical osteoin...
- Characterization of the nanoporous titanium surface
- Nanoporous titanium surface is biocompatible
- Cultivation of ITSCs on a titanium surface with 30nm pore size results in osteogenic differentiation without additional bio...
- ITSCs differentiated on a 30nm pore surface up-regulate osteogenesis-related transcripts
- Cultivation of ITSCs on the Ti surface equipped with 30nm pores elevates expression of Integrin subunits β1 and α2
- Cultivation of ITSCs on 30nm pores leads to increased phosphorylation of FAK at Y397
- ITSCs cultivated on flat Ti form diffuse actin fibers with weak polarity in contrast to well-defined polarized F-actin fibe...
- Discussion
- Conclusions
- Acknowledgments
- References