Stracke, Ralf ; Holtgräwe, Daniela ; Schneider, Jessica; Pucker, Boas ; Rosleff Sörensen, Thomas; Weisshaar, Bernd: Genome-wide identification and characterisation of R2R3-MYB genes in sugar beet ( Beta vulgaris ). In: BMC Plant Biology. Jg.14 H. 1. 2014
Inhalt
- Results and discussion
- Identification and genomic distribution of B. vulgaris R2R3-MYB genes
- Sequence features of the MYB domains
- Phylogenetic analysis of the B. vulgaris MYB family
- Expression profiles for B. vulgaris MYB genes in different developmental stages
- Functional analysis of the R2R3-MYB gene Bv_iogq
- Bv_iogq activates promoters of the flavonoid pathway structural genes
- Complementation of the flavonol-deficient phenotype of A. thaliana multiple pfg mutant
- Conclusions
- Methods
- Database search for MYB protein coding genes in the B.vulgaris genome
- Sequence conservation analysis
- Phylogenetic analyses
- Expression analysis from RNA-sequencing data
- Hierachical clustering and heatmap visualisation of RNA-seq data
- Plant material and growth conditions
- RNA isolation and cDNA synthesis
- cDNA cloning
- Complementation experiments with Bv_iogq
- Transient protoplast assays
- Availability of supporting data
- Additional files
- Competing interests
- Authors’ contributions
- Acknowledgements
- References