Kianianmomeni, Arash; Ong, Cheng Soon; Rätsch, Gunnar; Hallmann, Armin: Genome-wide analysis of alternative splicing in Volvox carteri. In: BMC Genomics. Jg.15 H. 1. 2014
Inhalt
- Results
- Genomic mapping of ESTs
- Alternative splicing analysis and abundance of splicing events
- Localization of alternative splicing events
- Experimental verification of alternative splicing events
- Discussion
- A comparative view on alternative splicing in Volvox
- Some striking alternative splicing events in Volvox
- Alternative splicing and organismal complexity
- Conclusions
- Methods
- Strain and culture conditions
- Data sources
- Genomic mapping of expressed sequence clusters
- Alternative splicing analysis
- Primer design
- Isolation of total RNA
- RT-PCR
- Quantitative real-time RT-PCR
- Additional file
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References