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Nolla Ardevol, Vimac; Strous, Marc; Tegetmeyer, Halina: Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome. In: Frontiers in Microbiology. Jg.6. 2015
Inhalt
Bioreactor Set-up
Analytical Methods
Determination of the Optimal Hydraulic Retention Time (HRT)
Determination of the Optimal Organic Loading Rate (OLR)
Operation at Optimal HRT and OLR Conditions
Metagenome and Metatranscriptome Analysis
DNA and RNA Extraction
DNA and cDNA Library Preparation
Metagenome Analysis
Removal of Spirulina Reads
Analysis of Sequencing Reads
Analysis of assembled reads
Phylogeny of assembled 16S rRNA sequences
Metatranscriptome Analysis
Functional Annotation of Selected Bins
Mapping of Transcripts
Analysis of Transcript Mappings
Detection of Genes and Transcripts Specific for Methanogenesis
Results
Biogas Rich in Methane
Determination of the Optimal Hydraulic Retention Time (HRT)
Determination of the Optimal Organic Loading Rate
Biogas Production at Optimal Operational Parameters
Parameters Affecting the Biogas Production
Binning and 16s rRNA Taxonomy Analysis of Assembled Contigs
Functional Analysis of the Selected Bins
Discussion
Biogas Rich in Methane
Biogas Production
Identification of Optimal HRT and OLR and Key Factors
Biogas Production at Optimal HRT and OLR
Identification of Key Players by the Taxonomic and Functional Analysis of the Haloalkaline Microbial Community
Hydrolysis and Uptake of Substrate
Methanogenesis
Microbial Structure of the Anaerobic Digestion Process at Alkaline Conditions
Conclusions and Perspectives
Author Contributions
Acknowledgments
Supplementary Material
References