Stadermann, Kai Bernd ; Weisshaar, Bernd ; Holtgräwe, Daniela: SMRT sequencing only de novo assembly of the sugar beet (Beta vulgaris) chloroplast genome. In: BMC Bioinformatics. Jg.16 H. 1. 2015
Inhalt
- Methods
- Plant material and DNA extraction
- Library construction and sequencing
- Raw sequencing data
- Extraction of potential chloroplast reads
- Assembly
- Creation of coverage plots
- Validating, reordering and polishing the assembly
- Annotation of the assembly
- Comparing the assemblies
- Results and discussion
- Subread extraction
- Result of the assembly process
- Comparison to the available sugar beet chloroplast assembly
- Annotation
- Conclusions
- Availability of supporting data
- Additional files
- Abbreviations
- Competing interests
- Authors’ contributions
- Authors' information
- Acknowledgements
- Author details
- References