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Nolla Ardevol, Vimac; Peces, Miriam; Strous, Marc; Tegetmeyer, Halina: Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads. In: BMC Microbiology. Jg.15 H. 1. 2015
Inhalt
Results and discussion
Biogas production via the anaerobic digestion of Spirulina
Metagenome analysis of the anaerobic digester community
Binning and 16S rDNA taxonomy analysis of assembled contigs
Comparison of two metagenomes by short single read analysis
Binning of contigs vs classification of single short reads
Conclusions
Methods
Bioreactor set-up
Analytical methods
Bioreactor adaptation and start-up
Metagenome analysis
DNA sample preparation, sequencing and quality trimming
Assembly of quality trimmed reads
In-depth taxonomy analysis
Phylogeny of assembled 16S rDNA sequences
Metagenome comparison
Accession numbers
Availability of supporting data
Additional files
Abbreviations
Competing interests
Authors’ contributions
Authors’ information
Acknowledgments
Author details
References