Stolze, Yvonne; Bremges, Andreas ; Rumming, Madis; Henke, Christian; Maus, Irena; Pühler, Alfred ; Sczyrba, Alexander ; Schlüter, Andreas: Identification and genome reconstruction of abundant distinct taxa in microbiomes from one thermophilic and three mesophilic production-scale biogas p [...]. In: Biotechnology for Biofuels. Jg.9 H. 1. 2016
Inhalt
- Methods
- Sampling at production-scale biogas plants and DNA extraction
- High-throughput 16S rRNA gene amplicon sequencing
- 16S rRNA gene sequence processing and quality control
- Operational taxonomic unit (OTU) clustering and taxonomic classification of 16S rRNA gene sequences
- Preparation and metagenome sequencing of total DNA from biogas-producing microbial communities
- Metagenome assembly and binning
- Results and discussion
- Parameters of the three mesophilic and one thermophilic industrial biogas plants analysed
- Sequencing results for 16S rRNA gene amplicons and metagenomes
- The microbiomes of one thermophilic and three mesophilic production-scale biogas plants determined by high-throughput 16S rRNA gene amplicon sequencing
- Similarities and differences of the microbiomes prevailing in the four analysed BGPs
- The phylum Thermotogae and its members are present only in the thermophilic BGP4
- The phylum Fusobacteria and its members are solely present in the mesophilic BGP3
- The phylum Spirochaetes and its members are most abundant only in the mesophilic plants BGP2 and BGP3
- The candidate phylum Cloacimonetes (WWE1) was only identified in the mesophilic biogas plants BGP2 and BGP3
- Most of the dominant distinctive taxa are not represented by closely related reference species in databases
- Reconstruction of genomes representing distinctive taxa applying metagenome assemblies and binning
- Metagenome assembly and binning results
- The genome bin representing a Thermotogae species from the thermophilic biogas plant 4
- The genome bin representing a Fusobacteria species from the mesophilic biogas plant 3
- The genome bin representing a Spirochaetes species from the mesophilic biogas plant 3
- The genome bins representing Cloacimonetes species from mesophilic biogas plants 2 and 3
- Conclusions
- Authors’ contributions
- References