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Höhn, Stephanie; Hallmann, Armin: Distinct shape-shifting regimes of bowl-shaped cell sheets – embryonic inversion in the multicellular green alga Pleodorina. In: BMC Developmental Biology. Jg.16 H. 1. 2016
Inhalt
Background
Results
Major movements of the cellular monolayer
Pre-inversion stage
Inversion and subsequent development
Region and stage specific changes in cell shape
Pre-inversion stage (Fig. 3a)
Early inversion stage (Fig. 3b, c)
Mid-inversion stage (Fig. 3d-f)
Late inversion stage (Fig. 3g-h)
Post-inversion stage (Fig. 4a-d)
Region and stage specific localization of CBs and other cellular details
Pre-inversion and early inversion stage
Mid-inversion stage
Late inversion stage
Post-inversion stage
Modeling of cell shapes, CB locations and illustration of the entire inversion process
Discussion
Hypotheses about intra- and inter-cellular forces during inversion of P. californica
Pre-inversion stage
Beginning of inversion - outward curling of the edge
Implementation of inversion - a wave of cell wedging
Post-inversion stage
Impacts of biomechanical constraints on the different inversion processes of multicellular volvocine algae
Evolution of inversion in volvocine algae
Conclusions
Methods
Strain and culture conditions
High-resolution in vivo stereo light microscopy
Fixation and dehydration of embryos
Staining
Light microscopy and transmission electron microscopy of sections
Two- and three-dimensional modelling
Additional files
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Acknowledgements
Funding
Availability of data and material
Authors’ contributions
Competing interests
Consent for publication
Ethics approval and consent to participate
Author details
References