Rafique, Muhammad Z.; Carvalho, Elisabete; Stracke, Ralf ; Palmieri, Luisa; Herrera, Lorena; Feller, Antje; Malnoy, Mickael; Martens, Stefan: Nonsense Mutation Inside Anthocyanidin Synthase Gene Controls Pigmentation in Yellow Raspberry (Rubus idaeus L.). In: Frontiers in Plant Science. Jg.7. 2016
Inhalt
- Plant Materials
- DNA and RNA Extraction
- Quantitative Real-Time PCR
- Cloning of Ans Gene
- Copy Number of Ans Gene in Rubus
- Transformation of Arabidopsis
- Inducing Anthocyanins in Arabidopsis Seedlings
- Establishment of Probe-Based Marker
- Results
- Expression Analysis of Anthocyanin Pathway Genes
- In silico Mining and Genomic Structure of Rubus Ans
- Sequence Analysis of Rubus Ans Gene
- Copy Number Analysis of Ans Gene
- Complementation of Arabidopsis ldox Mutant
- Development of DNA/RNA-Based Probe Marker
- Discussion
- Regulation of Gene Expression
- Molecular Analysis of Ans/ans Alleles
- Molecular Marker for Rubus Screening
- Complexity of Ans Gene
- Functional Characterization of Ans Gene
- Conclusion
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References