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Simonis, Matthias; Hübner, Wolfgang ; Wilking, Alice; Huser, Thomas ; Hennig, Simon: Survival rate of eukaryotic cells following electrophoretic nanoinjection. In: Scientific Reports. Jg.7 H. 1. 2017
Inhalt
Figure 1. Statistics of cell viability 24 hours after injection with two different voltages and timings.
Materials and Methods
Cells.
Fluorophores.
Figure 2. Time-lapse images of living U2OS cells injected with DAF.
Figure 3. Overlay of the wide-field fluorescence and whitelight images of single U2OS cells.
Pipettes and electrodes.
Setup.
Figure 4. Time-lapse fluorescence-/whitelight-imaging of a cell injected with DAF in a 100 nM SytoX Green Nucleic Acid Stain (SXG) environment.
Additional imaging.
Cell treatment and nanoinjection.
Figure 5. Statistics of cell status 24 hours after the injection of DAF with a 100 nm and 500 nm pipette into the nucleus and cytoplasm.
Acknowledgements
Author Contributions