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Lüdel, Frank: Self aggregation of IgG fragments from different perspectives. 2017
Inhalt
Contents
Summary
Acknowledgements
Introduction
Appendix
Basics of the Immune System
Basic principles
The immunoglobulins
Applications of antibodies and resulting requirements
The Aim of this Thesis
Numerical Methods and Software
CRYSOL
SASREF
HYDROPRO
Material and Methods
Small angle x-ray scattering
Light scattering
Size exclusion chromatography
Size exclusion chromatography - Hands on
Gel electrophoresis - SDS-PAGE
Gel electrophoresis - Hands on
Further parameters
Calculations
Results and Discussion
Initial cleaning and polishing of the 3H3 diabody
Light scattering
Small angle x-ray scattering
Calculations based on homologous protein structures
Calculation of the hydrodynamic data using HYDROPRO
Conclusion and Outlook
List of Publications
Bibliography
Used software
Chemical crosslinking
Sequence and generated sequence parts of 1LMK
Calculation of the hydroynamic properties of the protein after structure optimisation