Henke, Nadja Alina; Heider, Sabine; Hannibal, Silvin; Wendisch, Volker F. ; Peters-Wendisch, Petra: Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum. In: Frontiers in Microbiology. Jg.8. 2017
Inhalt
- Bacterial Strains, Media, and Growth Conditions
- Recombinant DNA Work
- Homologous Overexpression of Genes from C. glutamicum
- Deletion of crtR in C. glutamicum Strains
- Extraction of Carotenoids from Bacterial Cell Cultures
- Analysis of Carotenoids
- Transcriptome Analysis
- In vivo Measurement of Promoter Activities of crtR and crtE
- Overproduction and Purification of the Transcriptional Regulator CrtR
- Electrophoretic Mobility Shift Assay (EMSA)
- Results
- Co-occurrence of the MarR-Type Regulator Gene crtR with Genes of Carotenoid Biosynthesis in Completely Sequenced Actinobacterial Genomes
- CrtR Negatively Regulates Expression of the crt Operon and of Its Own Gene
- Gel Mobility Shift and Transcriptional Fusion Analyses Revealed That CrtR Binds to the Intergenic Region between crtR and crtE
- Isoprenoid Pyrophosphates Prevent Binding of CrtR to Its DNA Target
- Deletion of crtR Is a Metabolic Engineering Strategy to Increase Production of Native and Non-native Carotenoids
- Discussion
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References