Friehs, Karl; Schwarzhans, Jan Philipp; Luttermann, Tobias; Wibberg, Daniel; Winkler, Anika; Hübner, Wolfgang ; Huser, Thomas ; Kalinowski, Jörn: A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production. In: Frontiers in Microbiology. Jg.8. 2017
Inhalt
- Discovery of pMito
- Validation of Plasmid Character of pMito
- In Silico Analysis of pMito
- Transformation of pMito Variants into P. pastoris
- Productivity Characterization of pMito-I Strains
- Intracellular Localization of Expressed GFP
- mtDNA ARS from P. pastoris in S. cerevisiae
- Discussion
- Materials And Methods
- Microorganisms and Cultivation Conditions
- Genome Sequencing and Bioinformatic Analysis
- Construction and Transformation of Vectors
- In Silico Analysis of pMito ARS
- Plasmid Stability Assay
- Determination of GFP Expression Level and Gene Copy Number
- Super-Resolution Fluorescence Microscopy
- Author Contributions
- Acknowledgments
- Supplementary Material
- References