Kulis-Horn, Robert; Rückert, Christian ; Kalinowski, Jörn; Persicke, Marcus: Sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (HisN) in Corynebacterium glutamicum, Actinobacteria, [...]. In: BMC Microbiology. Jg.17 H. 1. 2017
Inhalt
- Background
- Results
- Genetic study on hisN and its four paralogs
- Enzymatic characterization of HisN and Cg0911
- Identification of sequence motifs for the discrimination of IMPase-like HolPases from other IMPase-like proteins in C. glutamicum and other bacteria
- Identification of IMPase-like HolPases based on the described sequence motifs and experimental validation of HolPase activity by complementation experiments
- Survey of the crystal structure of HisNZm focusing on the conserved HolPase motifs
- Distribution of HisN and Cg0911 orthologs within bacteria
- Discussion
- Conclusions
- Methods
- Bacterial strains and cultivation conditions
- Recombinant DNA work
- Gene deletion in C. glutamicum
- C. glutamicum ΔhisN complementation experiments
- Purification of the HisNCg and Cg0911 enzymes
- HolPase activity assay
- Bioinformatics analyses
- Additional files
- Abbreviations
- Acknowledgements
- Funding
- Availability of data and materials
- Authors’ contributions
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- References