Wolf, Timo; Droste, Julian; Gren, Tetiana; Ortseifen, Vera ; Schneiker-Bekel, Susanne; Zemke, Till; Pühler, Alfred ; Kalinowski, Jörn: The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. In: BMC Genomics. Jg.18 H. 1. 2017
Inhalt
- Background
- Results
- In silico analysis for the identification of a transcriptional regulator of the acarbose biosynthetic gene cluster and construction of a deletion mutant
- Establishment of whole genome microarrays for Actinoplanes sp. SE50/110 and application on a ΔacrC deletion mutant
- The transcription of the genes acbD and acbE is regulated by the repressor AcrC
- AcrC has an effect on the acarbose production
- The intergenic region between acbE and acbD features a binding site for AcrC
- Discussion
- Genetic engineering technologies and whole genome microarrays were established to characterize the transcription factor AcrC
- AcrC is the missing repressor of the acarbose biosynthetic gene cluster
- Conclusions
- Methods
- Strains, media and reagents
- Cultivation of Actinoplanes sp. SE50/110 and quantification of acarbose
- Construction of Actinoplanes sp. SE50/110 mutants
- Transcriptomic analyses
- Heterologous expression and purification of AcrC in Streptomyces lividans
- Electrophoretic mobility shift assays
- Additional files
- Abbreviations
- Funding
- Availability of data and materials
- Authors’ contributions
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- Author details
- References