Brouwer, Paul; Bräutigam, Andrea ; Buijs, Valerie A.; Tazelaar, Anne O. E.; van der Werf, Adrie; Schlueter, Urte; Reichart, Gert-Jan; Bolger, Anthony; Usadel, Björn; Weber, Andreas P. M.; Schluepmann, Henriette: Metabolic Adaptation, a Specialized Leaf Organ Structure and Vascular Responses to Diurnal N-2 Fixation by Nostoc azollae Sustain the Astonishing Productivity [...]. In: Frontiers in Plant Science. Jg.8. 2017
Inhalt
- Plant Materials
- Growth Conditions
- Confocal Laser Scanning Microscopy
- RNA Extractions and Quantitative RT-PCR
- RNA Sequencing and Bioinformatic Analyses
- N-Content Determinations and 15N2 Fixation Rates
- Results
- Azolla filiculoides Yielded 35 t DW ha-1 a-1 in an Experimental Continuous Harvest System
- Growth of A. filiculoides without N. azollae Entirely Relied on Exogenous N-Fertilizer
- N2-Fixation Rates Reached 0.4 mg N g-1dw h-1 at Noon and Dropped 7.5 Times in the Dark
- Transcriptional Patterns of the Diel Cycle Dominate over the Response to N-Fertilizer
- Transcriptional Profiles of Ferns Grown without N-Fertilizer Reflected Early Morning Recovery from Night-Time N Deficiency
- Pathways for the Assimilation of N into Amino Acids Remained Unchanged but Ureic Acid Metabolism Changed with N Supply
- Key Genes of the Vasculature Were Upregulated under –N Growth Conditions
- Discussion
- Light-Driven N2 Fixation by N. azollae Entirely Supported the High Yields of Azolla Biomass
- Azolla Yields without N-fertilizer Input Competed Favorably with Established Plant Protein Crops
- Specific Physiological Adaptations of the Ferns to the Diurnal Cyanobacterial N2 Fixation
- Features That Ensure Metabolic Connectivity between Host and Cyanobacteria
- The Evening Loop of the Clock is Ancestral to Ferns and Seed Plants
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References