Manandhar-Shrestha, K.; Tamot, B.; Pratt, E. P. S.; Saitie, S.; Bräutigam, Andrea ; Weber, Andreas P. M.; Hoffmann-Benning, Susanne: Comparative proteomics of chloroplasts envelopes from bundle sheath and mesophyll chloroplasts reveals novel membrane proteins with a possible role in [...]. In: Frontiers in Plant Science. Jg.4. 2013
Inhalt
- Plant material
- Isolation of mesophyll protoplast and BS strands
- Preparation of chloroplast envelopes
- Protein identification
- Data analysis
- Semiquantitative analysis of protein abundance
- RNA isolation and RT-PCR
- Subcellular localization
- Expression studies of chloroplast envelope proteins
- Results and discussion
- Identification and relative quantification of proteins associated with the envelope of BS and mesophyll chloroplasts
- Correlation of gene expression with protein abundance
- Confirmation of chloroplast localization of select chloroplast envelope proteins
- Distribution of gene expression throughout the plant and during transition to light
- Conclusion
- Acknowledgments
- Supplementary material
- References