Breuers, Frederique K. H.; Bräutigam, Andrea ; Geimer, Stefan; Welzel, Ulla Y.; Stefano, Giovanni; Renna, Luciana; Brandizzi, Federica; Weber, Andreas P. M.: Dynamic remodeling of the plastid envelope membranes - a tool for chloroplast envelope in vivo localizations. In: Frontiers in Plant Science. Jg.3. 2012
Inhalt
- Plant growth condition
- Cloning
- Agrobacterium tumefaciens preparation
- Agrobacterium tumefaciens mediated transformation of N. tabacum for in planta studies
- Agrobacterium tumefaciens mediated transformation of N. benthamiana for protoplast studies
- Protoplast isolation
- Microscopic analysis
- Transmission electron microscopy
- Results
- Distinct structures are observed when expressing AtTOC64 and AtTIC40 in transiently transformed tobacco cells
- Aberrant membrane structure formation is dependent on the protein amount
- Inner membrane alteration is independent of the protein properties
- LACS9 is a chloroplast outer envelope protein
- Experimental conditions do not affect the membrane alteration
- Investigating the identity of structural alterations
- Discussion
- Acknowledgments
- References