Franssen, Susanne U.; Shrestha, Roshan P.; Bräutigam, Andrea ; Bornberg-Bauer, Erich; Weber, Andreas P. M.: Comprehensive transcriptome analysis of the highly complex Pisum sativum genome using next generation sequencing. In: BMC Genomics. Jg.12 H. 1. 2011
Inhalt
- Results and Discussion
- The sequence read databases yielded 450 Megabases of sequence
- Two assemblies had very different properties
- The database annotation revealed low contamination and high redundancy
- While the transcripts were well covered, the transcriptome coverage was limited
- Normalization has unexpected effects on the sequence recovery
- The different libraries had different pathway representations
- Conclusions
- Methods
- Plant material and treatment
- RNA extraction
- cDNA library preparation
- Normalization of cDNA library
- EST pre-processing and assembly
- Unigene annotation
- Quantification of different sources contributing to redundancy between unigenes
- Mathematical analysis of library completeness
- Enrichment analysis
- Accession numbers of raw data
- Acknowledgements
- Author details
- Authors' contributions
- Competing interests
- References