Maus, Irena; Bremges, Andreas ; Stolze, Yvonne; Hahnke, Sarah; Cibis, Katharina G.; Koeck, Daniela E.; Kim, Yong S.; Kreubel, Jana; Hassa, Julia; Wibberg, Daniel; Weimann, Aaron; Off, Sandra; Stantscheff, Robbin; Zverlov, Vladimir V.; Schwarz, Wolfgang H.; König, Helmut; Liebl, Wolfgang; Scherer, Paul; McHardy, Alice C.; Sczyrba, Alexander ; Klocke, Michael; Pühler, Alfred ; Schlüter, Andreas: Genomics and prevalence of bacterial and archaeal isolates from biogas-producing microbiomes. In: Biotechnology for Biofuels. Jg.10 H. 1. 2017
Inhalt
- Methods
- Microbial strains used in this study and isolation of novel strains
- Phylogenetic classification of the analyzed bacterial and archaeal strains
- Genomic DNA extraction, sequencing, and bioinformatic analyses of biogas community members
- Prevalence of the investigated strains within microbial communities of four different agricultural biogas plants applying the metagenome fragment recruitment approach
- Results and discussion
- Selection of a set of microbial isolates from different biogas-producing communities
- Phylogenetic classification of the microbial isolates selected from different biogas communities
- Genome sequence analyses of the whole set of microbial isolates selected
- Screening of the subset of bacterial genomes to identify genes encoding carbohydrate-active enzymes potentially involved in biomass degradation
- Prediction of fermentation pathways based on sequence information for the subset of bacterial genomes
- Prediction of methanogenesis pathways based on sequence information for the subset of archaeal genomes
- Prevalence of bacterial and archaeal isolates in different microbial biogas communities analyzed by metagenome fragment mappings
- Conclusions
- Authors’ contributions
- References