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Hahn, Katharina; Hertle, Yvonne; Bloess, Svenja; Kottke, Tilman ; Hellweg, Thomas ; Fischer von Mollard, Gabriele: Activation of recombinantly expressed l-Amino Acid Oxidase from Rhizoctonia solani by Sodium Dodecyl Sulfate. In: Molecules. Jg.22 H. 12. 2017
Inhalt
Results
Effects of Detergents and Fatty Acids on 9His-rsLAAO1 Activity
9His-rsLAAO1 Behaved as a Dimer in the Native and SDS-Activated State
Indications for Conformational Changes in SDS-Activated 9His-rsLAAO1
Discussion
Materials and Methods
Expression and Purification of 9His-rsLAAO1
Enzyme Assay
Molecular Mass Determination by Size-Exclusion Chromatography
Photon Correlation Spectroscopy (PCS)
Circular Dichroism Spectroscopy
Fluorescence Spectroscopy
Reactivity of FAD with Sulfite