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Hattab, Georges ; Wiesmann, Veit; Becker, Anke; Munzner, Tamara; Nattkemper, Tim Wilhelm: A Novel methodology for characterizing cell subpopulations in automated time-lapse microscopy. In: Frontiers in Bioengineering and Biotechnology. Jg.6. 2018
Inhalt
2. Materials
3. Methods
3.1. Preprocessing
3.2. Particle Analysis
3.2.1. Particle Detection
3.2.2. Particle Trajectories
3.2.2.1. Particle Trajectory Linking
3.2.2.2. Particle Trajectory Filtering
3.2.2.3. Particle Trajectory Color Information
3.3. Patch Lineages
3.3.1. Algorithm Overview
3.3.2. Patch Finding
3.3.3. Patch Trajectory Propagation
3.3.4. Patch Trajectory Splitting
3.3.5. Patch Trajectory Merging
3.4. Computational Performance
3.5. Parameter Space
4. Implementation
5. Results
5.1. Preprocessing
5.1.1. Computational Performance
5.2. Particle Analysis
5.2.1. Spatial Coherence
5.2.2. Temporal Coherence
5.2.3. Computational Performance
5.2.4. Summary
5.3. Patch Lineage Graphs
5.3.1. Computational Performance
5.3.2. Summary
5.4. Biological Interpretation
6. Discussion
Author Contributions
Funding
Supplementary Material
References