Hartmann, Michael; Zeier, Tatyana; Bernsdorff, Friederike; Reichel-Deland, Vanessa; Kim, Denis; Hohmann, Michele; Scholten, Nicola; Schuck, Stefan; Bräutigam, Andrea ; Hoelzel, Torsten; Ganter, Christian; Zeier, Juergen: Flavin Monooxygenase-Generated N-Hydroxypipecolic Acid Is a Critical Element of Plant Systemic Immunity. In: Cell. Jg.173 H. 2. 2018, S. 456-469.e16
Inhalt
- Introduction
- Results
- Transcriptional Reprogramming by Pip is FMO1-Dependent and Constitutes an Integral Part of SAR
- Flavin-Dependent Monooxygenase1 Functions as a Pipecolic Acid N-Hydroxylase
- N-Hydroxypipecolic Acid Accumulates Systemically in the Arabidopsis Foliage at the Onset of Biological SAR
- NHP Generation in Arabidopsis Completely Depends on the Biosynthetic Genes ALD1 and FMO1 and Is Boosted by the Immune Regul ...
- Exogenous NHP Acts as a Potent Inducer of Plant Immunity to Bacterial and Oomycete Infection and Abolishes the Resistance D ...
- Discussion
- Supplemental Information
- Acknowledgments
- References
- STAR★Methods
- Key Resources Table
- Contact for Reagent and Resource Sharing
- Experimental Model and Subject Details
- Method Details
- GC-MS analysis of metabolites
- GC-FTIR analysis of metabolites
- Chemical synthesis of N-hydroxypipecolic acid
- Plant treatments with Pip and NHP
- Assessment of plant resistance to P. syringae
- Assessment of plant resistance to Hpa
- Trypan Blue Staining
- Microscopic analysis of Hpa infection
- Treatment with Isotope-Labeled Metabolites
- Cloning of FMO1
- Purification of recombinant FMO1 enzyme
- FMO1 activity assays
- Genome-Wide Analyses of the Plant Transcriptional Response to Pip
- Phylogenetic analysis of potential FMO1 orthologs
- Quantification and Statistical Analysis
- Data and Software Availability