Ruwe, Matthias; Rückert, Christian ; Kalinowski, Jörn; Persicke, Marcus: Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum. In: Frontiers in Microbiology. Jg.9. 2018
Inhalt
- Bacterial Strains and Growth Conditions
- Construction of C. glutamicum Deletion Mutants
- Constitutive Expression of RSH Genes in E. coli MG1655 and E. coli MG1655 relA
- Protein Purification
- Preparation of pGpp, ppGpp, and pppGpp
- (pp)pGpp Pyrophosphohydrolase Assay
- Bioinformatic Analyses
- Results
- The Genome of C. glutamicum Encodes a Putative Small Alarmone Hydrolase (SAH) Containing All Conserved (pp)pGpp Hydrolase Sequence Motifs
- The Deletion of relHCg Causes Only Minor Effects on the Growth Behavior of C. glutamicum Under Standard Growth Conditions
- RelHCg Exhibits a (pp)pGpp Pyrophosphohydrolase Activity in Vivo
- RelHCg Possesses a Mn2+ Dependent Pyrophosphohydrolase Activity in Basic pH Range in Vitro
- The Pyrophosphohydrolase Activities of RelHCg and RelCg Show Substrate Inhibition for Various Alarmone Species
- The Distribution of the relH Homolog in the Genus Corynebacterium Indicates a Frequent Gene Loss During Evolution
- Discussion
- Author Contributions
- Acknowledgments
- Supplementary Material
- References