Zumbrägel, Nadine ; Gröger, Harald: Merging Heterocyclic Chemistry and Biocatalysis in One-Pot Processes through Compartmentalization of the Reaction Steps. In: Bioengineering. Jg.5 H. 3. 2018
Inhalt
- Materials and Methods
- Preparation of the PDMS Thimbles for Compartmentalization of the Reaction Steps
- Construction and Expression of Whole-Cell Catalyst
- GC Analytics for the Determination of the Conversion
- Derivatization of 3-Thiazolidine (4a) with Phenylisocyanate
- HPLC Analytics for the Determination of the Enantiomeric Excess
- Biotransformation of 3-Thiazoline (3a) in the Presence of Different Amounts of Dimethyl Sulfoxide (DMSO)/3-Chloro-3-methylbutan-2-one (1)/Cyclopentanone (2)
- Studies on Diffusion of 3a through the PDMS Membrane
- Synthesis of 3-Thiazoline (3a) via Asinger-Reaction in Different Reactor Types
- One-Pot Process Combining the Asinger-Synthesis of 3-Thiazoline (3a) with an Enzymatic Reduction via Compartmentalization of the Reaction Steps
- Results and Discussion
- Compartmentation Approach
- Studies on Asinger-Type Multi-Component Reaction
- Diffusion Efficiency and Biocatalytic Reduction Using Imine Reductases
- One-Pot Process Combining the Asinger-Synthesis of 3-Thiazoline (3a) with an Enzymatic Reduction via Compartmentalization of the Reaction Steps
- Conclusions
- References