Whitford, Christopher M.; Dymek, Saskia; Kerkhoff, Denise; März, Camilla; Schmidt, Olga; Edich, Maximilian; Droste, Julian; Pucker, Boas ; Rückert, Christian ; Kalinowski, Jörn: Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications. In: Journal of Biological Engineering. Jg.12 H. 1. 2018
Inhalt
- Background
- Auxotrophy
- Strains auxotrophic for proteinogenic amino acids
- Strains auxotrophic for other natural components
- Evaluation of auxotrophic strains for biosafety purposes
- Auxotrophy systems in the iGEM competition
- Kill switch: plasmid retention and cell destruction
- Kill switch: DNA destruction
- Minimal genome
- Cell-free systems
- Physical containment
- Sensors
- Conclusion
- Abbreviations
- Acknowledgements
- Availability of data and materials
- Authors’ contributions
- Authors’ information
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- Author details
- References