Perez, Fernando; Jorge, João; Dreyszas, Annika; Risse, Joe Max ; Wendisch, Volker F.: Efficient production of the dicarboxylic acid glutarate by Corynebacterium glutamicum via a novel synthetic pathway. In: Frontiers in Microbiology. Jg.9. 2018
Inhalt
- Bacterial Strains, Vectors and Growth Conditions
- Molecular Biology Techniques
- Transcriptome Analysis
- Enzymatic Assay for GabT and GabD
- Quantitation of Carbohydrates, Organic Acids and Amino Acids by HPLC
- Glucose-Based Fed-Batch Fermentation
- Results
- Physiological and Genome-Wide Expression Response of C. glutamicum to Glutarate
- Metabolic Engineering for Efficient Provision of 5AVA for Glutarate Biosynthesis
- Establishment of Glutarate Production Using Different gabTD Operons
- Enforced Glutarate Production Upon Deletion of Glutamate Dehydrogenase gene gdh
- Bioreactor-Based Production of Glutarate in Fed-Batch Mode
- Discussion
- Author Contributions
- Acknowledgments
- References