Tian, Yuehui; Gao, Shiqiang; von der Heyde, Eva Laura ; Hallmann, Armin ; Nagel, Georg: Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases. In: BMC Biology. Jg.16 H. 1. 2018
Inhalt
- Results
- The “chlamyopsins” Cop1 and Cop2 are no membrane proteins
- Genes and mRNAs of Cr2c-Cyclop1 and Vc2c-Cyclop1
- Proteins of Cr2c-Cyclop1 and Vc2c-Cyclop1
- 2c-Cyclop proteins are membrane-embedded with cytosolic N- and C-termini and 8 predicted TM helices
- 2c-Cyclops are light-inhibited guanylyl cyclases
- Light regulation of 2c-Cyclop
- Photocycle turnover time of functional Cr2c-Cyclop
- Guanylyl cyclase activity of Cr2c-Cyclop1 under different reaction conditions
- Light-regulating mechanism inside Cr2c-Cyclop1
- Live cell imaging of Vc2c-Cyclop1 in V. carteri
- Discussion
- Conclusions
- Materials and methods
- Gene cloning, construction, and sequencing
- Xenopus oocyte membrane extraction and in vitro reaction
- Fluorescence emission detection
- Action spectra of Cr2c-Cyclop1 and Vc2c-Cyclop1
- Imaging of Xenopus oocytes
- Bioinformatics
- V. carteri strains and culture conditions
- Primer design
- Isolation of total RNA
- V. carteri transformation vectors
- Coating of microprojectiles for V. carteri transformation
- Stable nuclear transformation of V. carteri
- Quantitative real-time RT-PCR in V. carteri
- Sample preparation for cGMP concentration measurement in V. carteri
- cLSM analysis of YFP-tagged Vc2c-Cyclop1 in V. carteri
- Data processing
- Additional files
- Acknowledgements
- Funding
- Availability of data and materials
- Authors’ contributions
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- References