Borbély, Adina Noémi; Figueras Agustí, Eduard; Martins, Ana; Esposito, Simone; Auciello, Giulio; Monteagudo, Edith; Di Marco, Annalise; Summa, Vincenzo; Cordella, Paola; Perego, Raffaella; Kemker, Isabell; Frese, Marcel ; Gallinari, Paola; Steinkühler, Christian; Sewald, Norbert: Synthesis and Biological Evaluation of RGD–Cryptophycin Conjugates for Targeted Drug Delivery. In: Pharmaceutics. Jg.11 H. 4. 2019
Inhalt
- Materials and Methods
- Synthesis of Cryptophycin Conjugates
- Integrin Binding Assay
- Plasma Metabolic Stability Assay
- Cathepsin B Cleavage Assay
- Degradation in Human Liver Lysosomal Homogenate
- In Vitro Cytotoxicity Assay
- Flow Cytometry Analysis
- Confocal Microscopy
- Results and Discussion
- Design and Synthesis
- Integrin Binding Affinity
- Plasma Metabolic Stability
- Drug Release by Cathepsin B and Degradation in Human Liver Lysosomes
- In Vitro Cytotoxicity Assay
- Intracellular Uptake Studies
- Conclusions
- References