Simonis, Matthias; Sandmeyer, Alice; Greiner, Johannes; Kaltschmidt, Barbara; Huser, Thomas; Hennig, Simon: MoNa - a cost-efficient, portable system for the nanoinjection of living cells. In: Scientific reports. Jg.9 H. 1. 2019
Inhalt
- Results
- Table 1 Comparison of the MoNa and SICM-based injection systems.
- Figure 2 Comparison between the ion current during approach of a cell and the noise levels of the MoNa and SICM system (A,B) Ion current during approach and penetration of an U2OS cell with the MoNa system (current range: ±10 nA) (A) and the SICM system
- Discussion
- Figure 3 Nanoinjection of ATTO655-Phalloidin into the cytoplasm of a single living adult human inferior turbinate stem cell (ITSC) after neuronal differentiation (the injection point is indicated by the red arrow).
- Figure 4 Detailed view of the labeled ITC (Fig.
- Conclusions
- Figure 5 Overview of fluorescent probes and fluorophores which were successfully tested.
- Methods
- Cell Preparation.
- Isolation and culture of adult human inferior turbinate stem cells (ITSCs).
- Injection.
- Acknowledgements