Wegener, Melanie; Ennen, Inga; Walhorn, Volker; Anselmetti, Dario ; Hütten, Andreas; Dietz, Karl-Josef: Magnetic tracking of protein synthesis in microfluidic environments - challenges and perspectives. In: Nanomaterials. Jg.9 H. 4. 2019
Inhalt
- 1. Process and Significance of Protein Synthesis
- 2. Recent Progress in Studying Translation and Polysome Function: Need for Novel Analysis Tools
- 3. Magnetic Tracking of Protein Synthesis
- 3.1. Functionalization and Specific Labelling
- 3.2. Proposing a New Device for Separation and Detection Ensuring Further Analysis
- 3.3. Microfluidic Design and Physical Parameters
- 3.4. Challenges and Perspectives
- Appendix
- Material and Method A1: Isolation of Polysomes from Arabidopsis thaliana and Visualization with Transmission Electron Microscopy
- Material and Method A2: Pull Down of Polysomes from Arabidopsis thaliana with Functionalized Magnetic Particles
- Material and Method A3: Immunogold labelling of Polysomes from Arabidopsis thaliana
- References