Veldmann, Kareen; Dachwitz, Steffen; Risse, Joe Max ; Lee, Jin-Ho; Sewald, Norbert ; Wendisch, Volker F.: Bromination of L-tryptophan in a fermentative process with Corynebacterium glutamicum. In: Frontiers in Bioengineering and Biotechnology. Jg.7. 2019
Inhalt
- Bacterial Strains and Growth Conditions
- Determination of the Specific Activity of the Anthranilate Phosphoribosyltransferase TrpD
- Bioreactor Cultures Operated in Batch and Fed-Batch Mode
- Analytical Procedures
- Analytical RP-HPLC and RP-HPLC-MS
- NMR Spectroscopy
- High-Resolution MS
- Reversed-Phase Column Chromatography (GP1)
- Isolation and Purification of 7-Bromo-l-tryptophan From HSG Rich Medium
- Results
- Production of 7-Bromo-l-tryptophan in Flasks Culture
- Batch Production of 7-Bromo-l-tryptophan in a Bioreactor
- Fed-Batch Production of 7-Bromo-l-tryptophan in a Bioreactor
- Discussion
- Data Availability
- Author Contributions
- Funding
- Acknowledgments
- References