Mindt, Melanie; Hannibal, Silvin; Heuser, Maria; Risse, Joe Max ; Sasikumar, Keerthi; Nampoothiri, K.Madhavan; Wendisch, Volker F.: Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas [...]. In: Frontiers in Bioengineering and Biotechnology. Jg.7. 2019
Inhalt
- Materials and Methods
- Bacterial Strains and Culture Conditions
- Fed-Batch Bioreactor Process
- Preparation of Lignocellulosic Hydrolysates
- Molecular Genetic Techniques and Strain Construction
- Enzyme Preparation and Kinetic Characterization
- Quantification of Amines, Organic Acids, and Sugars in Fermentation Broth
- Results and Discussion
- Site-Directed Mutagenesis of the Active Site of the N-Methylamino Acid Dehydrogenase DpkA
- Expression of DpkAF117L in C. glutamicum Led to Enhanced Sarcosine and N-Ethylglycine Production
- Production of NEtGly From Lignocellulosic Hydrolysates
- Data Availability Statement
- Author Contributions
- Acknowledgments
- Supplementary Material
- References