Lüdel, Frank ; Bufe, Sandra; Bleymüller, Willem M.; de Jonge, Hugo de; Iamele, Luisa; Niemann, Hartmut ; Hellweg, Thomas: Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering. In: Antibodies. Jg.8 H. 4. 2019
Inhalt
- Materials and Methods
- Protein Expression and Purification
- Analytical Size Exclusion Chromatography
- Cell Motility Assay
- Small-Angle X-ray Scattering
- Photon Correlation Spectroscopy
- Static Light Scattering
- Molecular Mass Determination with SLS—The Zimm-Plot
- Density, Refractive Index and Refractive Index Increment Measurement
- Molecular Mass Calculation from SAXS Data
- Prediction and Evaluation of a Possible Structure Model
- Results and Discussion
- SEC Analysis
- SAXS Data Analysis
- Calculation of SAXS Data with CRYSOL
- Photon Correlation Spectroscopy (PCS) Data
- Calculation of Hydrodynamic Data with HYDROPRO
- Zimm Analysis
- Cell Motility Assay
- Conclusions
- References