Hassa, Julia; Wibberg, Daniel; Maus, Irena; Pühler, Alfred ; Schlüter, Andreas: Genome Analyses and Genome-Centered Metatranscriptomics of Methanothermobacter wolfeii Strain SIV6, Isolated from a Thermophilic Production-Scale Biogas [...]. In: Microorganisms. Jg.8 H. 1. 2020
Inhalt
- Materials and Methods
- Isolation of the Strain M. wolfeii SIV6 from the Thermophilic Biogas Fermenter
- Sequencing, Assembly and Annotation of the M. wolfeii SIV6 Genome
- Comparative Analyses of Methanothermobacter Genome Sequences
- Metatranscriptome Mapping
- Metagenome Fragment Recruitment
- Results and Discussion
- General Genome Features of M. wolfeii Strain SIV6
- Phylogenetic Classification as Deduced from Comparative Genome Analyses
- Genome Features of M. wolfeii SIV6 in Combination with In Situ Genome-Centered Metatranscriptomics
- Functional Genome Annotation in Combination with Transcriptional Activity of Genes
- Strain Specific Genome Features as Deduced from Singleton Analyses
- Genomic Islands and Restriction-Modification Systems as Additional Genome Features of M. wolfeii SIV6
- Reconstruction and Transcriptional Activity of the Hydrogenotrophic Methanogenesis Pathway of M. wolfeii SIV6
- Fragment Recruitment of Metagenomic Reads from the Corresponding Thermophilic Biogas Fermenter on the M. wolfeii SIV6 Genome
- Conclusions
- References